Conférence scientifique | Centre de recherche du CHU Sainte-Justine
Conférence de Jasmin Coulombe-Huntington, PhD, stagiaire postdoctoral, Institut de recherche en immunologie et en cancérologie (IRIC), Université de Montréal.
Genome-wide CRISPR/Cas9 knockout screens can reveal genotype-to-phenotype relationships and mechanisms of resistance and sensitization to drugs and other compounds. We have undertaken more than 250 CRISPR/Cas9 screens to map these relationships. I will present a plan to apply network-based approaches to model fitness effects of gene knockouts to predict drug mechanisms, gene functions, cancer-specific vulnerabilities and synergistic drug combinations. I will also cover earlier work on the network-level effects of alternative splicing and disease variants and present a machine learning-based strategy to reconstruct signaling pathways mediated by short linear motifs in disordered protein regions.