Titre : Single-Molecule Biosensors Using Nanoscale Electronic Devices.
Endroit : Pavillon Roger-Gaudry, salle G-615 à 11 h.
Hôte : Professeur André Charette.
Cette conférence sera prononcée (en français) par Delphine Bouilly, Ph.D., actuellement chercheuse postdoctorale dans le groupe du Prof. Colin Nuckolls au sein du Département de chimie de la Columbia University.
Résumé :
Organic macromolecules like proteins and nucleic acids regulate a wide variety of fundamental physiological processes, yet they are difficult to observe individually due to their small size, rapid fluctuations and complex environment. My research focuses on the design and application of single-molecule electronic biosensors, which are miniature electrical circuits capable to capture and probe individual biomolecules, in real time and over a uniquely-wide range of timescales. In this presentation, I will describe my results in developing original and robust single-molecule device architectures that are able to detect, through quantized fluctuations in electrical conductance, successive chemical reactions and conformational changes occurring on the same individual molecule. In particular, I will present recent experiments investigating the conformational dynamics of human telomeric DNA, by isolating an individual single-stranded DNA sequence and observing its repeated, reversible folding into a G‑quadruplex structure. Finally, I will discuss my plans to expand this emerging technique to explore other biomolecular mechanisms, for instance DNA hybridization, enzyme catalysis or protein folding, and to develop lab‑on‑chip technology for biomarker-based diagnosis and monitoring.
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